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. 2003 May;23(10):3636–3645. doi: 10.1128/MCB.23.10.3636-3645.2003

FIG. 5.

FIG. 5.

fNMHCB retains EXD in the cytoplasm of Drosophila S2 cells. Drosophila S2 cells were transfected with expression vectors for MEIS1A, GAL-DBD, and GAL-fNM individually or in combination. Immunostaining was performed with anti-EXD and anti-MEIS1 antibodies. DAPI staining reveals nuclei. (A and B) Endogenous EXD is located in the cytoplasm of S2 cells (which lack HTH, a Drosophila MEIS homologue). (C to E) Cotransfection of MEIS1A induces nuclear translocation of EXD. (F to H) GAL-fNM opposes the action of MEIS1A, inducing retention of EXD and MEIS1A in the cytoplasm.