FIG. 3.
Binding of GST-VP5 and GST-VP5D308A fusion proteins to cells in the presence of synthetic peptides and antibodies to VP5. (A) MA104 cell monolayers were incubated with 4 mg of the indicated peptides per ml for 1 h at 4°C, 1.5 μg of the purified recombinant proteins was added, and the cells were incubated for 1 h at 4°C. The amount of fusion protein bound to the cells was determined by an ELISA. Data are expressed as the percentage of protein bound to the cells when the cells were incubated with PBS as a control. (B) Recombinant GST-fusion proteins (1.5 μg) were incubated with the indicated MAbs for 1 h at room temperature, and this mixture was then added to monolayers of MA104 cells and incubated for 1 h at 4°C. The amount of bound protein was determined by an ELISA. Data are expressed as the percentage of protein bound to the cells when the proteins were incubated with PBS as a control. The arithmetic means and standard deviations for at least two independent experiments performed in duplicate are shown.