FIG. 4.

Binding of recombinant VP5-NH₂ and VP5-COOH proteins to cells in the presence of peptides and antibodies directed to cell surface molecules. MA104 cell monolayers were incubated with 4 mg of peptide per ml (A) or with antibodies against integrins α2β1 and α4β1 or to Hsc70 (B) for 1 h at 4°C. Then, 200 ng of purified recombinant VP5-NH₂ and VP5-COOH proteins was added and incubated for 1 h at 4°C. The amount of protein bound to the cells was determined by an ELISA. Data are expressed as the percentage of protein bound to the cells when the cells were incubated with PBS as a control. The arithmetic means and standard deviations for at least two independent experiments performed in duplicate are shown.