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. 2003 Jul;77(13):7563–7574. doi: 10.1128/JVI.77.13.7563-7574.2003

FIG. 3.

FIG. 3.

LC-derived mDCs support the complete replication cycle of human CMV strain TB40/E. (A to D) Colocalization of ppUL44 and IE1/IE2 by immunofluorescence analysis on cytospin preparations of LC-derived mDCs at day 3 p.i. (A) Phase-contrast photomicrograph of field; (B) IE1/IE2-positive nuclei (green); (C) ppUL44-positive nuclei (red); (D) merged image (panel B plus panel C). (E) The expression of the viral IE protein gpUS3 and the viral delayed early protein gpUS2 was monitored by immunoblot analysis of mock- or virus-infected HFs and LC-derived mDCs. Equivalent amounts of extracts from cells at day 2 p.i. were applied in each lane, and the presence of the two viral immunomodulatory proteins was detected with the polyclonal rabbit antisera US2N and US3N. (F) Single-step virus growth curve. At days 1, 5, 9, and 15 p.i., supernatant and cells from two separate wells were collected and sonicated and the titers were determined by plaque assay. Symbols represent virus titer values obtained from each of two separate wells at each time point.