TABLE 1.

Primers used for site-directed mutagenesis of U_L34^a

Charged cluster mutant	Primer sequence (5′→3′)	Restriction site introduced	Wild-type amino acid sequence	Mutant amino acid sequence
CL01	GAACGAGACCCgCGAAGGCGgCACCTGGGTGGCC	HphIb	GDAFE	GAAFA
CL02	GGGACGATAAGcgcAATTgcCTGAAGCAGACCCTCG	MfeI	QRIRL	QAIAL
CL03	CGTCCCATCTACGTTGgccGGCGGGGcCGGGGAGGCG	NgoMIV	LRGGD	LAGGA
CL04	ACGTTGCGGGGCGGGGcCGGGGctGCaGGCCCCTACTCTCCC	PstI	DGEAG	AGAAG
CL05	GGGAAACGACgCGgCGGACCCGgCATGGCCATGAAAC	PleIb	DGSDE	AGSAA
CL06	GCCCAGTCGTTCATAAGCgctAgcACATACgCGATGGGAAACG	NheI	IEYVL	IAYAL
CL07	ATCGAGTATGTACTGgcGCTTATGAACGcgTGGGCCGAGGTCCCG	MluI	RLMND	ALMNA
CL08	CTGTCCCTCGGCGcgCTaGcCACCATCAAGGGG	NheI	GDLDT	GALAT
CL09	GGCGACCTGGcCACCATCgcGGGGCGGCTCG	MscI	LDTIK	LATIA
CL10	CCTGGACACCATCgcGGGagcGCTCGGCCTGG	AfeI	IKGRL	IAGAL
CL11	CCATCAAGGGGgcGCTCGGCCTGGcgGCCCGGCCGATG	HaeII	RLGLD	ALGLA
CL12	GCGGCTCGGCCTGGcTGCggcGCCGATGATGGCCAG	KasI	LDARP	LAAAP
CL13	R:ATCAGCTGCTTTGTGgcCATGCCtgcaGTGCAGCTCGCGTTT	PstI	VRMPR	VAMPA
	L:CAAACGCGAGCTGCACtgcaGGCATGgcCACAAAGCAGCTGA
CL14	L:CAGGATCCGTCTCGTCgcTCCGGCtgCagCGGGGCCCATGAACCG	PstI	EDAGR	AAAGA
	R:CGGTTCATGGGCCCCGctGcaGCCGGAgcGACGAGACGGATCCTG
CL15	R:CCCGAAGATGCCGGAgcGACtgcAgcGATCCTGTGCCGCGCCG	PstI	GRTGG	GATAA
	L:CGGCGCGGCACAGGATCgcTgcaGTCgcTCCGGCATCTTCGGG
CL16	GCCGGACGGACGAGAgcGATCCTGTGCgcCGCCGCCGAGCAGGCT	BamHIb	RILCR	AILCA
CL17	R:GAGCAGGCTATTACCgcTgcCgcTgcAACCCGGCGGTCCCGG	HpyCH4IV	RRRRT	RRSRE
	L:CCGGGACCGCCGGGTTgcAgcGgcAgcGGTAATAGCCTGCTC
CL18	R:CGTCGCCGCCGAACCgcGgcGTCCgctGcaGCGTACGGGGCCGAG	PstI	RRSRE	AASAA
	L:CCGGCCTCGGCCCCGTACGCtcGagcGGACgcCgcGGTTCGGCGGCG ACGGGTAA
CL19	R:GCCGGAACGGGTTTCgcGGCtgcaGGGGcCGGTTTTGGCCCGCTC	PstI	RARGD	AAAGD
	L:GAGCGGGCCAAAACCGgCCCCtgcaGCCgcGAAACCCGTTCCGGC

^aLowercase letters indicate bases that differ from the wild-type HSV-1 sequence.

^bRestriction site was eliminated in the mutant.