FIG. 4.

Maintenance of latency (42 days) following i.n. infection is independent of the infectious dose. B6 mice were infected for 42 days and harvested as described in the legend to Fig. 3. (A) Ex vivo reactivation from latency. A limiting-dilution ex vivo reactivation assay was used to determine the frequency of peritoneal cells and splenocytes reactivating from latency as described in the legend to Fig. 1A. (B) Presence of viral genome in latently infected cells. A limiting-dilution nested PCR assay specific for γHV68 gene 72 was used to determine the frequencies of peritoneal cells and splenocytes containing viral genome as described in the legend to Fig. 2B. CPE, cytopathic effect.