FIG. 3.

Promoter activity of the APOC1 and EDNRB LTRs. Representation of the retroviral promoter constructs in which the APOC1- or EDNRB-associated LTRs were inserted upstream of the promoterless pGL3B vector and transiently transfected into the DLD-1, U87, A549, HepG2, 293, and Jeg-3 cell lines. The basic pGL3B vector and the simian virus 40 (SV40) promoter pGL3p plasmid were also transfected in the above cell lines. The luciferase activities obtained with each plasmid were corrected for transfection efficiency with the Renilla luciferase pRL-TK plasmid and are presented as increases (n-fold) over the activity of the basic (pGL3B) vector, which was assigned a value of 1. Each bar gives the mean of relative luciferase activity from at least 2 experiments ± standard deviation.