FIG. 8.

Mutational analysis of positions 175 to 215 of the EDNRB retroviral promoter. All constructs with mutations between positions 175 and 215 contain the full-length EDNRB LTR and were transfected in Jeg-3 cells. (A) In constructs Mut E, F, G, and H, the arrows represent nucleotides that have been replaced so as to have the same sequence as the APOC1 LTR. (B) In constructs Mut I, J, and K, X designates the putative transcription factor binding sites that have been removed by 3 to 5 mutations. (C) The sequence of nucleotides 175 to 215 in each construct is indicated, and the LPE2 motifs A, B, and C are boxed. Mutated nucleotides are shown in bold capital letters.