FIG. 3.
Termination efficiency of the nine natural HRSV gene junctions with L1049D or L1049N. HEp-2 cells, infected with MVA-T7, were transfected with dicistronic subgenomic replicons containing each of the naturally occurring HRSV gene junctions as indicated and support plasmids pP, pN, pM2ORF1, and pL1049D or pL1049N. Cells were labeled at 16 h posttransfection with [3H]uridine in the presence of actinomycin D. RNAs were purified, separated on an agarose urea gel, and visualized by fluorography. Percent readthrough at each gene end (left side) and percent termination efficiency (right side) were calculated for each replicon by densitometric analysis of the fluorographs. The average percent readthrough and standard deviation were calculated from at least three experiments.