TABLE 2.
Sensitivity and specificity of antibody and vRNA detection in feces and urine, respectivelya
| Detection method | HIV-1-infected chimpanzees
|
SIVcpz-infected chimpanzees
|
Uninfected chimpanzees
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Individuals | Samples | Sensitivity (95% CI) | Individuals | Samples | Sensitivity (95% CI) | Individuals | Samples | Specificity (95% CI)c | |
| ECL immunoblot (antibodies) | |||||||||
| Urine | 9/9 | 17/17 | 1.00 (0.84-1.00) | 3/3 | 10/10 | 1.00 (0.74-1.00) | 0/9 | 0/15 | 1.00 (0.82-1.00) |
| Feces | 12/5 | 26/42 | 0.62 (0.50-0.74) | 3/3 | 12/19 | 0.63 (0.47-0.83) | 0/23 | 0/51 | 1.00 (0.94-1.00) |
| RT PCR (vRNA) | |||||||||
| Urine | 1/9 | 1/17 | 0.06 (0.02-0.25) | 1/3 | 1/10 | 0.10 (0.04-0.39) | NDb | ND | 1.00 |
| Feces | 2/15 | 2/33 | 0.06 (0.02-0.18) | 4/4 | 14/25 | 0.56 (0.42-0.73) | ND | ND | 1.00 |
a
Sensitivity and specificity estimates are based on the number of samples rather than the number of individuals tested (each with 95% confidence interval [CI]).
b
ND, not done.
c
Specificity of vRNA detection by RT PCR is 100%, since all amplification products are sequence confirmed.