FIG. 3.

NF-κB translocation to HSV-1(F)-infected cell nuclei follows the immediate-early phase and is complete by the L phase of viral replication. HEp-2 cells were mock infected or infected with HSV-1(F) (MOI = 5), and at 3, 6, 9, and 12 hpi the infected cells were prepared for indirect immunofluorescence and double stained with antibodies specific for NF-κB (A and B) plus either IE ICP22 (A) or L VP22 (B) as described in Materials and Methods. Arrowheads in panel A mark a single cell with nuclear ICP22 and cytoplasmic NF-κB; in panel B, the arrowheads mark a cell with nuclear NF-κB and cytoplasmic VP22. Two representative microscopic fields are shown for each condition. Magnification, ×110.