FIG. 5.

NF-κB accumulation in HSV-1(F)-infected cell nuclei up to 12 hpi directly coincides with apoptosis prevention. HEp-2 cells were mock infected or infected with HSV-1(F) (MOI = 10), and CHX was added at 3, 6, 9, and 12 hpi. Mock-infected cells were treated with either CHX or CHX plus TNF-α at the time of infection. At 18 hpi, whole-cell extract (panel A) or cytoplasmic (C) and nuclear (N) extracts (panel B) were prepared, separated in denaturing gels, transferred to nitrocellulose membranes, and probed with anti-PARP (A) and anti-NF-κB (B) antibodies as described in Materials and Methods. 116 and 85 refer to full-length and processed PARP, respectively. “no tx” refers to cells that did not receive CHX or CHX-plus-TNF-α treatments. Locations of molecular mass markers (in kilodaltons) are indicated in the left margins.