TABLE 2.
New alanine-scanning mutagenesis of vD1-Ca
| vD1-C allele + vD12 | Growth at temp (°C):b
|
||
|---|---|---|---|
| 19 | 30 | 37 | |
| CEN | |||
| WTc | ++ | ++ | ++ |
| D604A | − | − | − |
| Y608A-F609A | − | − | − |
| D620A | − | − | − |
| R632A | − | − | − |
| F667A | ++ | ++ | ++ |
| F672A | ++ | ++ | ++ |
| D676A-W677A | − | − | − |
| E763A-Y764A | − | − | − |
| F775A | + | + | + |
| Y778A | ++ | ++ | ++ |
| F780A | + | + | − |
| D784A | + | + | − |
| F814A-F815A | − | − | − |
| 2μm | |||
| WT | +++ | +++ | +++ |
| D604A | − | − | − |
| Y608A-F609A | − | − | − |
| D620A | − | − | − |
| R632A | − | − | − |
| D676A-W677A | − | − | − |
| E763A-Y764A | − | − | − |
| F775A | +++ | +++ | +++ |
| F780A | ++ | ++ | ++ |
| D784A | +++ | +++ | + |
| F814A-F815A | + | + | + |
a
The indicated alleles of vD1-C were tested by plasmid shuffle for complementation of abd1Δ. CEN vD1-C plasmids were contransformed with CEN vD12 (CEN), and 2μm vD1-C plasmids were cotransformed with 2μm vD12 (2μm).
b
+++ indicates colony sizes indistinguishable from those of strains bearing wild-type ABD1, ++ indicates slightly reduced colony sizes, + indicates that only pinpoint colonies were formed, and − indicates no growth.
c
WT, wild type.