FIG. 2.

Affinity purification of a measles virus V protein-dependent complex. (A) Silver stain of the eluate from FLAG affinity purification. Migration positions of prestained molecular mass markers and FLAG-GFP, FLAG-SV5 V (SV), or FLAG-measles virus V (MeV) are indicated on the left in kilodaltons. SV5 VIP subunits are indicated on the right, as are prominent MeVIPs (indicated by asterisks). (B) Identification of MeVIP polypeptides. Parallel samples from the experiment in panel A were processed for immunoblotting with antisera for STAT1, STAT2, STAT3, IRF9, DDB1, or Cul4A.