FIG. 5.

Measles virus V protein prevents IFN-induced STAT nuclear accumulation. (A) Human 2fTGH cells were transfected with HA-tagged measles virus V cDNA and were untreated (UNT) or treated with IFN for 30 min 36 h later. The cells were stimulated with IFN-α (1,000 U/ml) to visualize STAT2 nuclear redistribution and IFN-γ (5 ng/ml) to visualize STAT1 nuclear redistribution. They were fixed, permeabilized, and stained with antisera to HA tag (red), and STAT1 or STAT2 (green). Merged images illustrate overlap in yellow; arrows point to V-expressing cells for clarity. (B) Same as in panel A, except that the cells were also treated with LMB (10 ng/ml) for 5 h.