FIG. 2.

Time course of RNA-dependent DNA synthesis catalyzed by HIV-1 and HIV-2 RTs. The RNA template contained nt 1 to 131 of HIV-1 genomic RNA (1). The reaction conditions, gel analysis, and quantification of the data by phosphorimaging are described in Materials and Methods and in Guo et al. (22). (A) Gel data. The time points are indicated above the lanes. The positions of (−) SSDNA, SP products (SP), and the primer are indicated to the left of the gel. (B) PhosphorImager analysis of gel data. Total DNA synthesized at each time point was determined by PhosphorImager analysis and was plotted as a function of time. Circles, HIV-1 RT; squares, HIV-2 RT.