TABLE 1.

FIV gag-specific primers and probe used in FIV fluorogenic Taqman real-time PCR assay^a

Primer or probe	Sequence (5′ to 3′)	Fragment size (bp)
Forward primer, FIV 1360f	GCA GAA GCA AGA TTT GCA CCA
Reverse primer, FIV1437r	TAT GGC GGC CAA TTT TCC T	78
Probe, FIV1416p	FAM-TGC CTC AAG ATA CCA TGC TCT ACA CTG CA-TAMRA
Forward primer, rRNA343f	CCA TCG AAC GTC TGC CCT A
Reverse primer, RRNA409r	TCA CCC GTG GTC ACC ATG	67
Probe, RRNA370p	FAM-CGA TGG TGG TCG CCG TGC CTA-TAMRA

^aCell number estimation was done using primers and probe targeting the 18S ribosomal DNA genes.