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Subtype C SI viruses have an increase in the number of basic amino acids compared to that in the env V3 loop consensus sequence (Con C) as well as a higher average net charge. The net charge of the sequence is derived from the summation of charges from positive and negative amino acids. A potential glycosylation site is underlined. Sequences for TC-03 and TC-28 correspond to virus that grew from MT2 cells; the remaining sequences are from low-passage-number PBMC stock.
