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Bmp1−/− Tll1−/− MEFs incorporate pC collagen forms, with uncleaved C-propeptides, and small amounts of apparently mature monomers into ECM. Western blots using antibodies directed against sequences in the proα1(I) C-telopeptide (A) or C-propeptide (B) demonstrate incorporation of pCα1(I) chains (A and B) and chains the size of mature, fully processed α1(I) chains into ECM associated with Bmp1−/− Tll1−/− MEF cell layers.
