TABLE 1.
Plasmids used in this study
| Activator or reportera | Plasmid | Note | Reference or source |
|---|---|---|---|
| Plasmids for in vitro transcription and translation | |||
| Bcd(WT) | pFY441 | SP6 promoter | 38 |
| Bed(92-489) | pFY7015 | SP6 promoter | 38 |
| Bed(1-246) | pFY443 | SP6 promoter | 38 |
| Bcd(92-246) | pFY7009 | SP6 promoter | This study |
| Plasmids for transient transfection | |||
| Bcd(WT) | pFY442 | Drosophila actin 5C promoter | 38 |
| Bcd(92-489) | pFY413 | Drosophila actin 5C promoter | 38 |
| Bcd(A52-56) | pFY436 | Drosophila actin 5C promoter | 38 |
| Bcd(A57-61) | pFY465 | Drosophila actin 5C promoter | 38 |
| Effector plasmids for yeast cells | |||
| Bcd(WT)-VP16 | pMA1226 | LEU2; yeast ADH1 promoter | 20 |
| Bcd(92-152)-VP16 | pCZ74 | LEU2; yeast ADH1 promoter | This study |
| Bcd(92-165)-VP16 | pCZ1007 | LEU2; yeast ADH1 promoter | This study |
| Bcd(92-205)-VP16 | pCZ1008 | LEU2; yeast ADH1 promoter | This study |
| Bcd(92-246)-VP16 | pCZ1010 | LEU2; yeast ADH1 promoter | This study |
| Bcd(1-246)-VP16 | pCZ71 | LEU2; yeast ADH1 promoter | This study |
| Bcd(12-246)-VP16 | pCZ1011 | LEU2; yeast ADH1 promoter | This study |
| Bcd(27-246)-VP16 | pCZ1012 | LEU2; yeast ADH1 promoter | This study |
| Bcd(42-246)-VP16 | pCZ93 | LEU2; yeast ADH1 promoter | This study |
| Bcd(1-151)-VP16 | pCZ32 | LEU2; yeast ADH1 promoter | This study |
| Bcd(1-165)-VP16 | pCZ69 | LEU2; yeast ADH1 promoter | This study |
| Bcd(1-205)-VP16 | pCZ70 | LEU2; yeast ADH1 promoter | This study |
| Reporter plasmids | |||
| hb-CAT | pCZ3005 | CAT reporter plasmid | 37 |
| kni-CAT | pCZ3006 | CAT reporter plasmid | 37 |
| kni(3R)-CAT | pDF520 | CAT reporter plasmid | This study |
| 3HH-CAT | pDF521 | CAT reporter plasmid | This study |
| 3TT-CAT | pDF522 | CAT reporter plasmid | This study |
| hb-lacZ | pMA630R | Integrating yeast reporter plasmid | 9 |
| kni-lacZ | pTA123 | Integrating yeast reporter plasmid | 37 |
a
WT, wild type.