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. 2003 Jul;23(13):4713–4727. doi: 10.1128/MCB.23.13.4713-4727.2003

FIG. 5.

FIG. 5.

p53-induced repression of cyclin D1 promoter activity can be prevented by Bcl-3 and the C terminus of p100. (A) Inhibition of cyclin D1 promoter activity by p53 can be reversed by expression of Bcl-3 or CT-p100. One and a half micrograms of cyclin D1-luciferase reporter plasmid was cotransfected with 1.5 μg of Bcl-3, CT-p100, or control RSV expression plasmid into H1299w/tp53 cells. p53 expression was induced by 100 μM IPTG, and cells were harvested 16 h later. Results are expressed as change in activation or repression (n-fold) relative to levels seen in the relevant untreated cell controls. luc, luciferase. (B) Inhibition of cyclin D1 promoter activity by p53 is not prevented by p52. One and a half micrograms of cyclin D1-luciferase reporter plasmid was cotransfected with 1.5 μg of p52 or control RSV expression plasmid into H1299w/tp53 cells. p53 expression was induced by 100 μM IPTG, and cells were harvested 16 h later. Results are expressed as change in activation or repression (n-fold) relative to levels seen in the relevant untreated cell controls.