FIG. 6.

GFP-PH-GRP1 protein localizes within and below the peak of the actin structure assessed by deconvolution analysis. L6 myoblasts were transiently transfected with 0.5 μg of plasmid encoding GFP-PH-GRP1 (a and b) or GFP-PH(K273A)-GRP1 (c and d), stimulated with insulin, and stained for F-actin as described in the legend for Fig. 4. Images from several focal planes (optical sections of 0.2 μm) were obtained, deconvolved, and volume rendered to obtain a three-dimensional reconstruction of the cell. The red signal (F-actin) is reduced to reveal the green signal (GFP) below (b and d). Bar, 10 μm.