FIG.5.
Late-gestation fetal livers of nrf1lacZ/lacZ chimeric mice show increased apoptosis and necrosis. (a and b) Livers of E18.5 chimeric fetuses showing patches of necrotic tissue. (c) Liver shown in panel b that was whole mount stained with X-Gal. (d) Cross section of E18.5 liver showing X-Gal staining in hepatocytes. (e) Cross section of E18.5 liver stained with hematoxylin and eosin. Note the presence of pkynotic and fragmented nuclei (red arrows in panels d and e). (f) TUNEL showing apoptotic cells. (g) Section of an E18.5 chimeric liver showing the border between healthy (upper left) and necrotic (lower right) tissues. (h) Higher-magnification photomicrograph of an E18.5 fetal liver section showing clusters of nucleated erythroid cells (red arrows) scattered between necrotic tissue.