TABLE 1.

Changes in gene expression identified by quantitative RT-PCR in nrf1^−/− fetal livers and erythroid progenitors^a

Gene	Fold changeb (mean ± SD)
	Fetal liver	CFU-E from ES cells
Gclc	−2.9 ± 1.1	0.3 ± 0.4
Gclm	−3.7 ± 1.2	−0.3 ± 0.5
Gpx-1	−5.1 ± 1.7	ND
MT-1	−4.0 ± 2.0	0.3 ± 0.3
MT-2	−3.0 ± 2.0	ND
HO-1c	−2.1 ± 1.3	NT

^aThe levels of expression of genes in each sample were quantitated relative to endogenous GAPDH levels as an internal reference. Expression levels relative to GAPDH levels were calculated as follows: 2(Ct of the test gene − Ct of the GAPDH gene).

^bThe change in expression of the various genes was determined from the difference between the average expression levels of wild-type and Nrf1 mutant samples. Decreases are indicated by minus signs before the values. For fetal liver comparisons, the livers of five wild-type and six nrf1^−/− mice were used. For comparisons of CFU of erythroid cells (CFU-E) from ES cells, cells from two wild-type and three nrf1^−/− mice were used. ND, not determined; NT, not detected.

^cHO-1, heme oxygenase 1.