FIG. 5.

Double Bax/Bak deficiency abolished sensitivities toward apoptosis in GBM. Bax⁺/Bak⁺ (BeGBM), Bax⁻/Bak⁺ (BdGBM), Bax⁺/Bak⁻ (antisense Bak-transfected BeGBM cells) and Bax⁻/Bak⁻ (antisense Bak-transfected BdGBM) cells were treated with UV irradiation (10 s to 120 s) and increasing concentrations of DOXO (up to 20 ng/ml) or STS (up to 20 μM). Cells were transfected with increasing concentrations of plasmid encoding human C3 (up to 20 μg of plasmid) or an empty plasmid (20 μg) used as a control. It should be noted that no significant cell death was recorded in mock-transfected cells after selection. Cell death was quantified after 24 h using the release of LDH (measured as described in Materials and Methods) in the cell culture medium. Representative results from one of 3 independent experiments are illustrated for each treatment.