FIG. 2.

Differential pattern of expression of Nck1 and Nck2 in adult tissues. The expression of Nck1 and Nck2 was assessed in various tissue extracts from Nck1^−/− Nck2^−/− mutants and WT mice. Equivalent amounts of tissue extracts (determined by Bio-Rad assay) were probed with the 3x3 polyclonal antibody, recognizing the first SH3 domain of Nck1 and Nck2. Since this region was deleted in our targeting strategies, we used tissues from Nck2^−/− and Nck1^−/− mutants to assess the expression of Nck1 and Nck2, respectively. Membranes were stripped and reblotted with anti-β-tubulin antibody. Arrowheads mark the position of the indicated protein bands. The results are representative of two independent experiments.