FIG. 9.
Actin network organization upon ATP recovery. Platinum replica electron microscopy of lamellipodia of Nck1+/− Nck2+/−, Nck1−/− Nck2−/−, and Nck1-rescued MEFs after a 1- or 20-min recovery period from ATP depletion was completed. One minute after sodium azide washout, the recovery of Nck1−/− Nck2−/− MEFs was comparable to that of Nck1+/− Nck2+/− and Nck1-rescued MEFs. In contrast, after 20 min, the recovery of Nck1−/− Nck2−/− MEFs was significantly impaired compared to that of Nck1+/− Nck2+/− and Nck1-rescued MEFs. Nck1−/− Nck2−/− MEFs had shorter filaments at the leading edge and less-branched filaments behind the leading edge.