TABLE 2.

H1 subtype composition and total H1 stoichiometry per nucleosome in wild-type and H1 compound-knockout mice^a

Tissue and genotype	% of total H1b						Total H1/nucleosomec (no. of determinations)
	H1°	H1a	H1b	H1c	H1d	H1e
Thymus
Wild type (+/+)	1.9 ± 1.6	10.7 ± 1.3	16.5 ± 0.9	26.7 ± 3.5	35.4 ± 2.7	9.6 ± 1.5	0.83 ± 0.10 (12)
H1°−/−; H1cH1e−−/−−	0	17.0 ± 3.5	28.3 ± 3.0	0	54.7 ± 5.5	0	0.47 ± 0.26 (8)
H1cH1dH1e−+−/−−−	5.9 ± 4.6	25.0 ± 3.0	35.4 ± 3.1	0	31.7 ± 2.6	0	0.41 ± 0.16 (6)
Liver
Adult
Wild type (+/+)	29.0 ± 0.8	1.4 ± 0.6	15.8 ± 3.5	12.5 ± 1.0	14.3 ± 0.6	39.9 ± 2.5	0.79 ± 0.07 (5)
H1°−/−; H1cH1e−−/−−	0	2.2 ± 0.3	6.3 ± 1.3	0	91.5 ± 1.6	0	0.64 ± 0.01 (4)
Neonatal
Wild type (+/+)	9.5 ± 4.9	6.1 ± 2.4	14.2 ± 1.7	20.9 ± 4.5	30.3 ± 2.3	19.0 ± 1.5	0.76 ± 0.07 (5)
H1cH1dH1c−+−/−−−	26.7 ± 6.5	9.9 ± 2.2	29.3 ± 4.6	0	32.7 ± 5.2	0	0.50 ± 0.15 (6)

^aData are from HPLC analyses of wild-type and H1 compound-knockout mice. Values are means ± standard deviations of individual determinations made on each of the indicated genotypes.

^bDetermined by ratio of A₂₁₄ of the indicated H1 peak to the total A₂₁₄ of all H1 peaks. The A₂₁₄ values of the individual H1 peaks were adjusted to account for the differences in the number of peptide bonds in each H1 subtype.

^cDetermined by the ratio of the total A₂₁₄ of all H1 peaks to half of the A₂₁₄ of the H2b peak. The A₂₁₄ values of the H1 and H2b peaks were adjusted to account for the differences in the number of peptide bonds in each H1 subtype and H2b.