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Methylation patterns for the expressing MCa1 and derived silenced cells. (A) CpG sites analyzed were divided into four regions (see text for details). Arrows represent the B1-5 and B1-6 repetitive elements within the methylation center. CpG sites 13 and 16 are located within Sp1 binding sites 3 and 4, respectively. There is no CpG site 10 on this construct. (B to D) Bisulfite sequencing analyses to identify methylated (closed triangles) and unmethylated (open triangles) CpG sites.
