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Activated Akt1 enhances complex formation between p300 and bHLH proteins. P19 cells were transfected with expression vectors for Myc epitope-tagged ngn3 (MT-ngn3) (A) or MT-MASH1 (B) with and without constitutively active Akt1. Endogenous p300 was immunoprecipitated from extracts 24 h after transfection and subjected to SDS-PAGE. p300 and coassociated Myc epitope-tagged bHLH proteins were detected by Western blot analysis with antibodies directed against p300 or the Myc epitope tag.
