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. 2003 Jul;23(13):4417–4427. doi: 10.1128/MCB.23.13.4417-4427.2003

FIG. 5.

FIG. 5.

Activated Akt1 enhances the transcriptional activity of ngn3 in complex with the transcriptional coactivators p300 and CBP. P19 cells were transfected with expression vectors for the Gal4-DBD p300 fusion protein (Gal-p300) or Gal4-DBP-CBP fusion protein (Gal-CBP), ngn3, the pFR Gal4-responsive luciferase reporter, and constitutively active Akt1 (C/A Akt) or constitutively active Sgk1 (C/A SGK). The PI3K/Akt inhibitor LY294002 (12.5 μM) was added at the time of transfection, as indicated. (A) ngn3 and C/A Akt1 cooperatively enhance the transcriptional activity of Gal-p300. (B) ngn3 and C/A Akt1 cooperatively enhance the transcriptional activity of Gal-CBP. (C) Inhibition of the endogenous PI3K/Akt signaling pathway with LY294002 decreases the transcriptional activity of Gal-p300 in combination with ngn3, but not in the presence of C/A Akt1. (D) C/A Akt1, but not C/A SGK, enhances the transcriptional activity of ngn3 and Gal-p300.