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Summary of purification of d-HYD from E. coli BL21(DE3)/pXZPH2
| Purification step | Sample vol (ml) | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purifi- cation factor | Activity yield (%) |
|---|---|---|---|---|---|---|
| Cell extract | 25.0 | 600 | 30.8 | 0.05 | 1.0 | 100 |
| Ni-NTAa affinity | 4.0 | 4.4 | 5.0 | 1.13 | 22.6 | 16.6 |
NTA, nitrilotriacetic acid.
