TABLE 2.

Summary of active site electrostatic interactions and hydrogen bonding in PCK-Ca²⁺-Mg²⁺-ATP-pyruvate complex

Substrate	Coordinating group	Distance (Å)
Ca2+	Nɛ2 H232	2.6
	Oδ1 D269	2.5
	Oδ2 D269	3.0
	O1γ ATP	2.4
	H2O 719	2.6
	O1α PVT	2.8
	Nζ K213	2.7
Avg		2.7
Mg2+	O2β ATP	2.1
	O3γ ATP	2.2
	Oγ T255	2.1
	H2O 674	2.3
	H2O 716	2.5
	H2O 738	2.3
Avg		2.2
PVT
O1α	Ca2+	2.8
	H2O 719	3.2
O1β	65 NH2	2.8
O2	H2O 719	3.4