TABLE 1.
Primers used in this studya
| Primer | Sequence (5′ to 3′) |
|---|---|
| Primers for upstream sites with a HindIII restriction site | |
| Pri−177F | TCTGCCTGTAAGCTTAATGGAAGAGCCTATTTTTATGG |
| Pri−143F | GAAAAAAAGCTTAATGGGTCAATTCAGATCG |
| Pri−123F | ATTCAGAAGCTTCCGTGCGGGAAAAAAATCG |
| Pri−91F | GTATTTGAAGCTTAATTGATCTAAAACACG |
| Pri−83F | ATTCAGAAGCTTAAAACACGAACATTAGTAG |
| Pri−75F | TGATCTAAAGCTTGAACATTAGTAGAATGAATTTTTG |
| Pri−69F | AAACACGAAGCTTAGTAGAATGAATTTTTG |
| Pri−54F | AGAATGAAGCTTTGTATCGTTCGATAATATCG |
| Primers for downstream sites with an EcoRI restriction site | |
| Pri+24R | TCCCAGAATTCTATGAGGTCGTGTTTTG |
| Pri−6R | TTCAGAATTCTCTTAACAACGGACATG |
| Pri−31R | CATGGATGAATTCAACGATATTATCGAACG |
| Pri−42R | CAACGGAATTCTCGAACGATACAAAAATTC |
| Pri−60R | ATACAAGAATTCATTCTACTAATGTTCGTG |
| Pri−75R | CTACTAGAATTCGTGTTTTAGATCAATTTC |
a
Restriction sites are underlined.