TABLE 2.

Estimation of molecular size in solution by DLS^a

Protein and bufferb	Total mass (%)	RH (nm)	CP/RH (%)	RH range (nm)c	Subunits per oligomer	Predominating subunits (oligomer range)	Baseline error
cpn10Mt
A	99.99	3.70	10.4	3.51-3.89	8.0,d 5.9e	7	1.012
B	95.9, 4.1	4.31, 36.77	29.8, 54.1	3.67-4.95	13.3f	14 (7-14+)	1.001
GroES
C	99.9	3.70	10.2	3.51-3.89	8.0,d 5.9e	7	1.003
D	99.7, 0.3	4.27, 29.10	24.8, 21.2	3.74-4.80	12.9f	14 (7-14+)	1.003

^aVolume shape hydration (VSH) models with hydrodynamic radii (R_H) calculated from crystal structure data. R_H for cpn10_Mt heptamer without base loops, 3.54 nm; R_H for cpn10_Mt heptamer, 3.92 nm; R_H for cpn10_Mt tetradecamer, 4.39 nm; R_H for GroES heptamer without base loops, 3.49 nm.

^bBuffers: A, 2.3 mg of cpn10_Mt per ml in 100 mM TrisHCl (pH 6.8), 50 mM KCl; B, 2.65 mg of cpn10_Mt per ml in 40 mM sodium acetate (pH 5.4), 8 mM CaCl₂; C, 0.56 mg of GroES per ml in 40 mM sodium acetate (pH 7.4); D, 0.5 mg of GroES per ml in 36 mM sodium acetate (pH 7.4), 20 mM CaCl₂.

^cEstimated from polydispersity.

^dCorresponding to VSH model a.

^eCorresponding to VSH model b.

^fCorresponding to VSH model c.