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CD spectra of human Cpn10 (2 μM) in MeOH- or TFE-100 mM KH2PO4 mixtures (1:1, vol/vol) (A) and of E. coli Cpn10 (0.1 μM) in 166 mM KH2PO4-MeOH (0.3:0.7, vol/vol) at 25° and 40°C (B).