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CD spectra of monomeric M. tuberculosis Cpn10 in the presence of increasing concentrations of MeOH (A) and TFE (B). The organic solvents were added to a solution of protein in KH2PO4, pH 7.4. The concentration of KH2PO4 in these solvent mixtures was kept fixed at 50 mM, and that of the protein was kept at 4 μM.
