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Elution volume as a function of analyte molecular weight (MW) for columns B0, packed with beads without PMEA graft (□) and B1, packed with beads carrying terminally attached chains (■). Solutes loaded are: sodium nitrate (log MW = 1.9), myoglobin (log MW = 4.2), human serum albumin (log MW = 4.8), and keyhole limpet hemocyanin (log MW = 6.5).
