Figure 4.
Measured and calculated partition coefficients as a function of protein molecular weight for columns C1 (■) and C2 (□). Characteristics of both columns are provided in Table 1. Proteins are: insulin (5.8 kDa), myoglobin (17.2 kDa), trypsin inhibitor (21.5 kDa), ovalbumin (43 kDa), human serum albumin (66 kDa), and BSA (68 kDa). Calculations are based on SCF theory in a cylindrical geometry for surface-graft densities of 2% (C1 column) and 10% (C2 column). Model scaling was based on polymer and protein radius of gyration data, resulting in approximate tether coverages of 1.5 groups/Å2 (upper curve) and 6 groups/Å2 (lower curve); χij as in Fig. 1.