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Amplified expression and purification of OpuA. (A) SDS/PAGE gel (12% polyacrylamide) showing membranes (Coomasie brilliant blue stained) containing OpuAA (lane 1; 120 μg of protein), OpuA (lane 2; 120 μg of protein), OpuABC (lane 3; 120 μg of protein), and purified OpuA (silver stained) (lane 4; 15 μg of protein). (B) Immunoblot of membranes with overexpressed OpuA (lane 1; 120 μg protein), OpuABC (lane 2; 120 μg protein), and OpuAA (lane 3; 250 μg protein).
