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Nuclear extracts from several cultured cell lines show DQA1 Y-box-specific binding activity. Oligonucleotide probes containing the DQA1*0101 Y-box sequence (lanes 1–7) or DQA1*0501 polymorphic Y-box sequence (lanes 8–12) bind protein in nuclear extracts prepared from resting cells of the indicated cultured cell lines. Lane 1, control without nuclear extract; lanes 2 and 8, 2.5 μg of protein; lanes 3–7 and 9–12, 5 μg of protein.
