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NZO-3 binds directly to CZO-3. (A) Coomassie Blue-stained gel showing equal amounts of GST and GST-NZO-3 present on the beads. (B) After the addition of purified histidine-tagged CZO-3 to GST and GST-NZO-3 beads, the bound fraction was probed with an antibody specific for the C-terminal half of ZO-3. CZO-3 is retained on the NZO-3–containing beads. A small amount of nonspecific binding occurs with GST alone.
