Figure 2.

Small localized wounds in the squid fin nerve and giant axon. Different size wounds were made in a fin nerve of the squid with either a 25× 0.8 NA multi-immersion lens (A–C) or a 63× 1.2 NA (D–F) water immersion lens. Arrowheads point to the wound in the transmitted light images A and D. The corresponding fluorescent scar is shown in the x-y (C and F) and y-z (B and E) planes. The corresponding volumes for these fluorescent scars are 76, 10.9, and 376.1 μm³ (B and C) and 10.9 and 0.253 μm³ (E and F). (G) Spot wound in the squid giant axon using a 63× 1.2 NA water immersion lens. To confirm that damage was confined to the wound plane axonal transport was monitored by taking time series of Rhodamine 123 labeled mitochondria at the wound plane, 4 μm above and 4 μm below the wound plane (see online movies: above.mov and below.mov). The volume of the wound was 13 μm³. Arrowheads indicate the wound site in the out of plane images. Scale bar for all images, 10 μm.