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. 2000 Jun 6;97(13):7112–7117. doi: 10.1073/pnas.130187897

Figure 4.

Figure 4

SDS/PAGE (A) and Western blots (B) of MP incubated with trypsin showed that a core domain resists cleavage. Lanes 1, MP inclusion bodies (MPIB) in TNEM8MU buffer. Lanes 2, P1, and lanes 3, P2 (see Fig. 2A). Lanes 4–14, MP from P2 in TNETMG buffer. Lanes 4, control incubation, no trypsin (NT). Lanes 5–14 were incubated with trypsin for the indicated times, in min. Mr as in Fig. 1; m, monomer; c, core. Ponceau-S staining showed even transfer of proteins to membranes. Trypsin (<2 ng per lane) was not detected in the gels.