Table 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| BY85 | MATa/α ura3-52/ura3-52 leu2-3, 112/leu2-3, 112 his3Δ200/his3Δ200 YPT1/YPT1ΔHIS3 | Brennwald laboratory |
| BY86 | MATa/α ura3-52/ura3-52 leu2-3, 112/leu2-3, 112 his3Δ200/his3Δ200 SEC4/SEC4ΔHIS3 | Brennwald laboratory |
| AG6 | LEU2::SEC4(L7,HV)YPSEC4Δ::HIS3 his3Δ200 ura3-52 | Brennwald laboratory |
| BY24 | MATa/α ura3-52/ura3-52 leu2-3, 112/leu2-3, 112 | Brennwald laboratory |
| NY605 | MATaura3-52 leu2-3, 112 | Novick laboratory |
| RCY1507 | MATα ura3-52 leu2-3, 112 his3Δ200 SEC4ΔHIS3 [YCP50 SEC4] | This study |
| RCY1510 | ura3-52 leu2-3, 112 his3Δ200 YPT1ΔHIS3 [pRS316 (pRC1762) YPT1] | This study |
| RCY 1530 | MATa/α ura3-52/ura3-52 leu2-3, 112/leu2-3, 112 YIP1/YIP1ΔKANR [YCP50 (pRC1245) YIP1] | This study |
| RCY1610 | MATaura3-52 leu2-3, 112 YIP1ΔKANR [YCP50 (pRC1245) YIP1] | This study |
| RCY1764 | MATaura3-52 leu2-3, 112 YIP1ΔKANR [pRS315 yip1-4] | This study |
| RCY1760 | MATaura3-52 leu2-3, 112 YIP1ΔKANR [pRS315 YIP1] | This study |
| Y190 | MATagal4Δ gal80Δ trp1-901 ade2-101 ura3-52 leu2-3,112 URA3::GAL10 → LacZ, LYS2::GAL10 → HIS3 cyhR | Elledge laboratory |