Figure 2.

ykt6 mutants are temperature sensitive for growth and CPY sorting. (A) Wild-type (SEY6210), ykt6-11 (YKY10), ykt6-12 (YKY5), and ykt6-13 (YKY11) cells were transformed with either a low copy plasmid carrying YKT6 (bottom) or an empty vector (top). The transformants were streaked on YEPD plates and incubated for3dat either 30°C (left) or 37°C (right). The growth defects are suppressed by the presence of the YKT6 plasmid. (B) Indicated strains were labeled with [³⁵S]methionine for 10 min at 25°C or for 10 min at 37°C after a 15-min preincubation at 37°C, and chased for 45 min. CPY was immunoprecipitated from intracellular (I) and extracellular (E) fractions and analyzed by SDS-PAGE and autoradiography.