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GFP-ALP mislocalization in ykt6-13 cells is overcome by overexpression of Nyv1p. ykt6-13 (YKY11) cells carrying a plasmid expressing GFP-ALP were transformed with an empty, or a multicopy plasmid with either YKT6 or NYV1. GFP-ALP was visualized by fluorescence microscopy after cell growth at 30°C.
