Figure 7.

A DNA repair-defective mutant (irs1SF) shows extra centrosome-like structures spontaneously during mitosis that can result in the formation of aneuploid cells. Time-lapse confocal analysis of irs1SF cells expressing γ-tubulin–GFP. Several of the mitoses recorded spontaneously showed the formation of extra centrosome-like structures during mitosis, and some of the cells with extra centrosome-like structures finally formed more than two daughter cells (arrows) as shown here. Time points are indicated in minutes. Bar, 10 μm.