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. 2003 May;14(5):2005–2015. doi: 10.1091/mbc.02-07-0102

Figure 2.

Figure 2.

F-actin cytoskeleton is altered and focal complexes increased in cyclin D1-/- BMMs. Cyclin D1 WT or cyclin D1-/- BMMs were plated on fibronectincoated coverslips and stimulated with CSF-1 for 30 min after over-night removal of CSF-1 (A–D) or cultured in the continuous presence of CSF-1 (E and F). Cells were stained for F-actin (A and B), phosphotyrosine (C and D), and β-tubulin and cyclin D1 (E and F), before examination by cooled CCD microscopy (A–D) or confocal microscopy (E and F) (60×).