Table 2.

xbx-1 expression analyses using an in vivo GFP expression assay

Genotype	Wild type			daf-19(m86)
Strength of Expression	Strong	Weak	Absent	Strong	Weak	Absent
xbx-1::gfp
line 1	86	14	0	1	37	62
line 2	66	31	3	1	33	66
line 3	61	33	6	0	22	78
line 4	51	41	8	0	3	97

Data are given as percent expression in ciliated sensory neurons in the head of adult animals. Only the ciliated sensory neurons adjacent to the nerve ring were examined. To classify expression as “strong,” “weak,” or “absent,” the number of neurons expressing xbx-1::gfp as well as the visibility of GFP in neuronal cell bodies and in processes extending to the tip of the nose were taken into consideration. Four independent transgenic lines were analyzed. Each row represents the data for one transgenic line. For each transgenic line (containing the wild-type X-box promoter element sequence) the transgene was also moved from wild type into a daf-19 mutant background. Eighty to 120 animals were analyzed for each transgenic line.